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Spectrofluorimetric investigation for determination of sumatriptan succinate: application to tablets and spiked human plasma
Author(s) -
Abo Zaid Mona H.,
Abo El abass Samah,
ElEnany Nahed,
Aly Fatma
Publication year - 2021
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.4000
Subject(s) - detection limit , chromatography , human plasma , sumatriptan , chemistry , biochemistry , receptor , agonist
A simple and highly sensitive spectrofluorimetric method for the estimation of sumatriptan succinate has been investigated. The suggested method depends on the determination of the intrinsic fluorescence properties of the drug in aqueous systems at λ em 350 nm following λ ex at 225 nm. The linearity range was 10–100 ng/ml, with a detection limit and quantitation limit of 1.2 and 3.6 ng/ml, respectively. The suggested method was sufficiently successful for determination of sumatriptan its pharmaceutical tablets as well as in spiked human plasma. Moreover, the validation parameters were determined following International Council for Harmonisation guidelines. Statistical analysis of the obtained results from the proposed and reference methods showed no significance difference between the two methods regarding accuracy and precision.