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Flow injection–chemiluminescence determination of cetirizine dihydrochloride in pharmaceuticals using tris(2,2 ′‐ bipyridyl)ruthenium (II)–Ag(III) complex reaction
Author(s) -
Moazzam Muhammad,
Asghar Muhammad,
Yaqoob Mohammed,
Ali Samar,
Nabi Abdul
Publication year - 2021
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.3985
Subject(s) - ruthenium , chemistry , chemiluminescence , detection limit , sulfuric acid , tris , potassium hydroxide , chromatography , flow injection analysis , nuclear chemistry , analytical chemistry (journal) , inorganic chemistry , catalysis , organic chemistry , biochemistry
A simple and sensitive flow injection–chemiluminescence (FI–CL) method was developed for determination of cetirizine dihydrochloride (CTZH) in pharmaceuticals. The method is primarily based on the enhancement effect of CTZH on the tris(2,2′‐bipyridyl)ruthenium (II)–diperiodatoargentate (III) ([Ru(bpy) 3 ] 2+ –Ag(III) complex) CL system in an acidic medium. The optimum investigated variables of the CL reaction were: [Ru(bpy) 3 ] 2+ , 50 × 10 −6 mol/L; sulfuric acid, 1.0 × 10 −3 mol/L; Ag(III) complex, 100 × 10 −6 mol/L; potassium hydroxide, 1.0 × 10 −3 mol/L; flow rate, 3.0 ml/min and sample loop volume, 300 μl. The detection and quantification limits were 2.0 × 10 −4 and 5.0 × 10 −4 mg/L ( S/N of 3 and 10) respectively with a linear calibration range of 5.0 × 10 −4 to 7.5 mg/L ( R 2 = 0.9999, n = 11), injection throughput of 110/h and the relative standard deviations of 1.5–3.5% over the range studied. The methodology was successfully applied to determine CTZH in different pharmaceutical samples and validated with a high‐performance liquid chromatography method, and resulted in the recovery of 94.6–108.6%. The probable CL reaction mechanism is described in brief.