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Characterization of a novel styrylbenzimidazolium‐based dye and its application in the detection of biothiols
Author(s) -
Kolińska Jolanta,
Grzelakowska Aleksandra
Publication year - 2021
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.3956
Subject(s) - chemistry , fluorescence , hela , quantum yield , glutathione , cytotoxicity , photochemistry , mass spectrometry , nuclear chemistry , maleimide , cysteine , detection limit , proton nmr , chromatography , stereochemistry , organic chemistry , in vitro , biochemistry , physics , quantum mechanics , enzyme
A novel styrylbenzimidazolium dye containing a maleimide group 5 was synthesized and characterized using proton nuclear magnetic resonance spectroscopy and mass spectrometry. The photophysical properties [ultraviolet–visible (UV–vis) light absorption and fluorescence spectra, fluorescence quantum yield, and fluorescence lifetime] were investigated. Spectroscopic characterization of the novel styrylbenzimidazolium‐based dye under various conditions is presented and its usefulness to detect biothiols proved. The addition of biothiols [ l ‐cysteine ( l ‐Cys), l ‐homocysteine ( l ‐Hcy), l ‐glutathione ( l ‐GSH)] to compound 5 in phosphate buffer (0.1 M, pH 7.4) containing 10% CH 3 CN induced a 15–28‐fold enhancement in fluorescence intensity at 410 nm. The limits of detection of compound 5 for l ‐Cys, l ‐Hcy, and l ‐GSH were estimated as 0.114, 0.118, and 0.059 μM, respectively. Evaluation of the cytotoxicity of 5 using the PrestoBlue assay for HeLa cells was also determined. The examined compound revealed a slight cytotoxicity against HeLa cells under experimental conditions.