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A caged 2‐hydroxyethyl luciferin for bioluminescence imaging of nitroxyl in living cells
Author(s) -
Ju Yong,
Wang Anni,
Li Xuewei,
Xu Xu,
Lu Jianzhong
Publication year - 2020
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.3902
Subject(s) - luciferin , nitroxyl , bioluminescence , bioluminescence imaging , chemistry , biophysics , luciferase , radiochemistry , photochemistry , biochemistry , biology , transfection , gene
Nitroxyl (HNO), a one‐electron reduction product of nitric oxide, demonstrates distinct biological and pharmacological activities. Here we designed a bioluminescent turn‐on probe, HNO‐8, that could be used to visualize HNO without the need for excitation light. HNO‐8 was prepared by caging 2‐hydroxyethyl luciferin with a triphenylphosphine unit, in which 2‐hydroxyethyl luciferin as a novel substrate of firefly luciferase was characterized by stronger and more sustained bioluminescent signals than the most popular substrates of d ‐luciferin and 6′‐aminoluciferin. In vitro experiments showed that HNO‐8 could selectively respond to HNO generated from Angeli's salt(AS) in the range 1–50 μM, with a limit of detection of 0.196 μM. The probe was successfully applied for visualizing HNO in luciferase‐transfected Huh7 cancer cells. We envision that HNO‐8 could be used as a powerful bioluminescent sensor for researching HNO biological roles.