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Two water‐soluble fluorescence probes based on 5(6)‐carboxyl rhodamine for Cu 2+ imaging in living cells
Author(s) -
Zhang Mengyao,
Wang Jun,
Jia Ting,
Qiu Jianwen,
Zhu Hu,
Gao Yong
Publication year - 2020
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.3822
Subject(s) - fluorescence , rhodamine , titration , chemistry , acetonitrile , metal ions in aqueous solution , buffer solution , detection limit , fluorescence lifetime imaging microscopy , rhodamine b , metal , analytical chemistry (journal) , nuclear chemistry , inorganic chemistry , chromatography , organic chemistry , catalysis , physics , quantum mechanics , photocatalysis
Two novel water‐soluble fluorescence probes T1 and T2 based on 5(6)‐carboxyl rhodamine were designed and synthesized using a regioselective reaction. The probes exhibited highly selective and sensitive recognition toward Cu 2+ over other metal ions in acetonitrile/Tris–HCl buffer solution (2:98, v /v; pH 7.4). Detection limits were 0.4 μM for T1 and 4.50 μM for T2 based on fluorescence titration analysis. Furthermore, probe T 1 was successfully applied in cell imaging experiments to monitor Cu 2+ in cells.