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A coumarin‐based fluorescent probe for Hg 2+ and its application in living cells and zebrafish
Author(s) -
Li Xiwei,
Duan Qingxia,
Yu Yamin,
Wang Kun,
Zhu Hanchuang,
Zhang Xue,
Liu Caiyun,
Jia Pan,
Li Zilu,
Sheng Wenlong,
Zhu Baocun
Publication year - 2020
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.3808
Subject(s) - mercury (programming language) , fluorescence , chemistry , detection limit , metal ions in aqueous solution , zebrafish , selectivity , intramolecular force , coumarin , ion , metal , photochemistry , environmental chemistry , chromatography , stereochemistry , organic chemistry , biochemistry , physics , quantum mechanics , gene , computer science , programming language , catalysis
Mercury (Hg) is a heavy metal with high toxicity and easy migration; it can be enriched through the food chain, and cause serious threats to the natural environment and human health. So, the development of a method that can be used to detect mercury ions (Hg 2+ ) in the environment, in cells, and in organisms is very important. Here, a new 7‐hydroxycoumarin‐derived carbonothioate‐based probe ( CC‐Hg ) was designed and synthesized for detection of Hg 2+ . After addition of Hg 2+ , a large fluorescence enhancement was observed due to the formation of 7‐hydroxyl, which reinforced the intramolecular charge transfer process. The CC‐Hg probe had good water solubility and selectivity. Moreover, the probe was able to detect Hg 2+ quantitatively over the concentration range 0–2 μM and with a detection limit of 7.9 nM. Importantly, we successfully applied the probe to detect Hg 2+ in water samples, in living cells, and in zebrafish. The experimental results demonstrated its potential value in practical applications.