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Condensation of heptaminol hydrochloride for its spectrofluorimetric determination in pure form and tablets: application in human plasma
Author(s) -
Halim Monica E.,
Omar Mahmoud A.,
Nagy Dalia M.
Publication year - 2020
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.3787
Subject(s) - detection limit , reagent , calibration curve , chemistry , formaldehyde , chromatography , condensation , moiety , derivative (finance) , hydrochloride , linear range , fluorescence , correlation coefficient , condensation reaction , human plasma , analytical chemistry (journal) , organic chemistry , mathematics , thermodynamics , catalysis , physics , statistics , quantum mechanics , financial economics , economics
A sensitive, simple, accurate and less expensive fluorimetric method was designed and validated for analysis of heptaminol HCl in both its pure and dosage forms, as well as in human plasma. The main principle used in the proposed approach was the condensation reaction between heptaminol's primary amino moiety and ethyl acetoacetate/formaldehyde reagents, giving a derivative that was highly fluorescent at 416 nm after excitation at 350 nm. Various experimental parameters that affected either the product's development or its stability were evaluated and optimized. The constructed calibration curve was linear over the range 0.2–2 μg/ml, with a good correlation coefficient (0.9996). Both the calculated limit of detection and limit of quantitation were 0.06 and 0.18 μg/ml, respectively. The presented approach was a success when used to determine Corasore® tablets and was validated according to International Council for Harmonisation guidelines.