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Redox‐modulated colorimetric detection of ascorbic acid and alkaline phosphatase activity with gold nanoparticles
Author(s) -
Lin Liping,
Luo Yaxin,
Chen Qiushuang,
Lai Qingjiao,
Zheng Qiaoling
Publication year - 2020
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.3749
Subject(s) - chemistry , ascorbic acid , colloidal gold , absorbance , detection limit , surface plasmon resonance , nuclear chemistry , alkaline phosphatase , redox , colorimetry , chromatography , nanoparticle , inorganic chemistry , enzyme , biochemistry , materials science , nanotechnology , food science
Gold nanoparticles (AuNPs) exhibit characteristic absorption peaks in the ultraviolet visible region due to their special surface plasmon resonance effect. This characteristic absorption peak would change with the relative colour varying from wine red to orange‐yellow upon sequential addition of ascorbic acid (AA) into the mixture of AuNPs and Ag(I). Similar observations also could be found when the hydrolysis product of sodium l ‐ascorbyl‐2‐phosphate with alkaline phosphatase (ALP) was used as an alternative to AA. Results of structure characterization confirmed that the phenomena were due to the reduction of Ag(I) to Ag(0) on the surface of AuNPs and the formation of core‐shell AuNPs@Ag. Therefore, a colorimetric assay for rapid visual detection of AA and ALP based on redox‐modulated silver deposition on AuNPs has been proposed. Under the optimal experimental conditions, the absorbance variation ΔA 522 nm /A 370 nm of AuNPs was proportional to the concentration of AA (5–60 μmol/L) and ALP (3–18 U/L) with the corresponding detection limit of 2.44 μmol/L for AA and 0.52 U/L for ALP. The assay showed excellent selectivity towards AA and ALP. Moreover, the assay has been applied to detect AA and ALP activity in real samples with satisfying results.