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Assessment of the potential bactericide effect of self‐cleaning floors: a proposed protocol
Author(s) -
SzabóBárdos Erzsébet,
Kulcsár Petra,
Kováts Nóra,
Békéssy Zsófia,
EckVaranka Bettina,
Horváth Ottó
Publication year - 2020
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.3745
Subject(s) - ecotoxicity , bioassay , suspension (topology) , bioluminescence , coating , protocol (science) , chemistry , environmental chemistry , environmental science , biology , medicine , toxicity , mathematics , organic chemistry , biochemistry , alternative medicine , pathology , genetics , homotopy , pure mathematics
The antibacterial properties of self‐cleaning coatings are based on bactericide nanoparticles (NPs). Ecotoxicity of these NPs have been assessed mostly in suspension, using standard bioassays. Here a protocol is proposed to test actual coating samples, using the Vibrio fischeri bioluminescence inhibition bioassay. The protocol was designed to test bactericide properties of specially coated PVC floors being used in hospital environments under quasinatural conditions, such as prolonged exposure or room temperature. To take into consideration that the light output of the bacteria under prolonged exposure naturally changes, a correction factor is proposed.