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Synthesis and activity of a coumarin‐based fluorescent probe for hydroxyl radical detection
Author(s) -
Zhang Bin,
Xu Lijing,
Zhou Yindi,
Zhang Weijian,
Wang Yuanhong,
Zhu Yu
Publication year - 2020
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.3728
Subject(s) - chemistry , coumarin , hydroxyl radical , fluorescence , reactive oxygen species , glutathione , scavenging , photochemistry , selectivity , oxidative stress , isopropyl alcohol , metal ions in aqueous solution , radical , metal , antioxidant , organic chemistry , biochemistry , catalysis , physics , quantum mechanics , enzyme
As a type of reactive oxygen species (ROS), hydroxyl radical (·OH) is closely associated with many kinds of diseases. The present study aimed to develo p a novel OH fluorescent probe based on coumarin, a new compound that has not been previously reported. This probe exhibited good linear range and selectivity for ·OHl, and is able to avoid interference from some metal ions and other kinds of ROS (H 2 O 2 , O 2 .‐ , 1 O 2 , and HClO). Meanwhile, this probe has been used to evaluate the ·OH‐scavenging efficiency of different compounds, such as isopropyl alcohol, cytosine, uracil, Tempo, Glutathione (GSH), and dimethyl sulfoxide (DMSO). Therefore, the present study shows that this probe not only can effectively measure the level of ·OH, but also can assess the ·OH‐scavenging efficiency of different compounds. Furthermore this current study suggested that following further optimization, this probe may be potentially applied in the diagnosis of oxidative stress in human body.