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Hg 2+ ‐selective ratiometric and colorimetric probe based on dansyl–rhodamine and its staining function in cell imaging
Author(s) -
Wang Yuesong,
Ding Haichang,
Wang Shuai,
Fan Congbin,
Tu Yayi,
Liu Gang,
Pu Shouzhi
Publication year - 2019
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.3690
Subject(s) - absorbance , chemistry , fluorescence , rhodamine , dansyl chloride , detection limit , hydrazide , rhodamine b , acceptor , photochemistry , analytical chemistry (journal) , chromatography , derivatization , catalysis , organic chemistry , high performance liquid chromatography , physics , quantum mechanics , photocatalysis , condensed matter physics
A new ratiometric probe composed of a dansyl–rhodamine dyad for the detection of Hg 2+ via fluorescence resonance energy transfer was designed and synthesized. Rhodamine, dansyl chloride, and hydrazide were selected as the acceptor, donor, and reaction site, respectively. It displayed high selectivity and sensitivity to Hg 2+ with obvious colour change and fluorescence change due to Hg 2+ ‐assisted hydrolysis of rhodamine hydrazide. A good linear relationship ranging from 0 to 16 μM and 0–28 μM for the Hg 2+ concentration was found based on absorbance and fluorescence assay, respectively. Detection limits of absorbance and fluorescence for Hg 2+ were calculated to be 1.22 μM and 9.10 μM, respectively.