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Simple ultrasensitive spectrofluorimetric method for determination of midodrine in its tablet form: Application to content uniformity testing
Author(s) -
Omar Mahmoud A.,
Nagy Dalia M.,
Halim Monica E.
Publication year - 2019
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.3682
Subject(s) - detection limit , calibration curve , chromatography , linearity , midodrine , calibration , analytical chemistry (journal) , content determination , materials science , chemistry , mathematics , high performance liquid chromatography , statistics , physics , medicine , quantum mechanics , blood pressure , orthostatic vital signs , radiology
A novel, simple, sensitive method was developed for determining midodrine spectrofluorimetrically in both its raw pure form and its tablet form. This study is based on the native fluorescence of midodrine and discusses the optimum solvent used and the wavelength range. The presented method was then validated with respect to linearity, accuracy, precision, and limits of detection and quantitation. The constructed calibration curve showed a linear range of 0.1–2.0 μg/ml. The limit of detection and limit of quantitation values were 0.03 and 0.10 μg/ml respectively. Finally, content uniformity testing was applied according to the United States Pharmacopoeia by adapting the presented method.