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Signal‐on fluorescence assay for pyrophosphate ions based on DNA‐stabilized silver nanoclusters
Author(s) -
Xu Huifeng,
Yu Lishuang,
Zhang Shiqi,
Xu Xueya,
Chen Tingting,
Ye Hongzhi,
Zhu Xi
Publication year - 2019
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.3673
Subject(s) - pyrophosphate , nanoclusters , detection limit , fluorescence , chemistry , calcium pyrophosphate , ion , quenching (fluorescence) , copper , photochemistry , chromatography , calcium , biochemistry , organic chemistry , physics , quantum mechanics , enzyme
Pyrophosphate anion (P 2 O 7 4− , PPi) is considered as a potential biomarker for arthritic diseases because high levels of PPi may result in calcium pyrophosphate dehydrate crystal deposition diseases. In this study, a simple fluorescence method for PPi was demonstrated by organic integration of the efficient fluorescence quenching ability of copper ions to DNA‐scaffolded silver nanoclusters and the strong affinity of PPi towards copper ions. This simple fluorescence sensor showed a low detection limit (0.28 μM based on signal/noise = 3) towards the detection of PPi. Practical application of this method was also validated by detection of PPi in the synovial fluid.