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Establishment of an EC 50 database of pesticides using a Vibrio fischeri bioluminescence method
Author(s) -
Efremova Aaron Snezana,
TosheskaTrajkovska Katerina,
Cekovska Svetlana,
Aaron JeanJacques
Publication year - 2019
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.3628
Subject(s) - pesticide , bioluminescence , vibrio , toxicity , environmental chemistry , chemistry , population , pollutant , toxicology , bacteria , biology , food science , biochemistry , ecology , medicine , organic chemistry , genetics , environmental health
An EC 50 database was established to assess the acute toxicity of 16 PESTANAL pesticide standards and of seven pesticide commercial formulations using a Vibrio fischeri bioluminescence method. Half maximal effective concentration ( EC 50 ) is defined as the concentration of pollutant (in this case, pesticide) destroying 50% of the bacteria population and causing 50% bioluminescence inhibition, after a specified exposure time. Linear curves of bioluminescence inhibition versus pesticide concentration and EC 50 values were obtained for exposure times ( t ) of 5 or 15 min for these pesticides. The EC 50 values ranged from 6.90 × 10 −4 to 0.83 mg/ml ( t = 5 min), and from 9.00 × 10 −4 to 0.37 mg/ml ( t = 15 min) for pesticide standards, plus from 0.0077 to 0.74 mg/ml ( t = 5 min), and from 0.0076 and 0.57 mg/ml ( t = 15 min) for pesticide commercial formulations. The EC 50 database allowed classification of the pesticides under study into three categories according to their toxicity: very toxic, toxic and moderately toxic. These results demonstrated that the establishment of an EC 50 database and of linear curves of bioluminescence inhibition versus the pesticide concentration resulted in very important and irreplaceable tools to estimate the global and individual toxicity of pesticides present in environmental samples.