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A reversible calix[4]arene armed phenolphthalein based fluorescent probe for the detection of Zn 2+ and an application in living cells
Author(s) -
Erdemir Serkan,
Malkondu Sait,
Kocyigit Ozcan
Publication year - 2019
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.3585
Subject(s) - phenolphthalein , fluorescence , chemistry , detection limit , aqueous solution , zinc , photoinduced electron transfer , metal ions in aqueous solution , photochemistry , electron transfer , ion , analytical chemistry (journal) , chromatography , organic chemistry , physics , quantum mechanics
A reversible and easy assembled fluorescent sensor based on calix[4]arene and phenolphthalein (C4P) was developed for selective zinc ion (Zn 2+ ) sensing in aqueous samples. The probe C4P demonstrated high selective and sensitive detection towards Zn 2+ over other competitive metal ions. Interaction of Zn 2+ with a solution of C4P resulted in a considerable increment in emission intensity at 440 nm ( λ ex = 365 nm) due to the suppression of photoinduced electron transfer (PET) process and the restriction of C=N isomerization. The binding constant ( K a ) of C4P with Zn 2+ was calculated to be 4.50 × 10 11 M −2 and also the limit of detection of C4P for Zn 2+ was as low as 0.108 μM (at 10 −7 M level). Moreover, the fluorescence imaging in the human colon cancer cells suggested that C4P had great potential to be used to examine Zn 2+ in vivo .