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Colorimetric detection of gallic acid based on the enhanced oxidase‐like activity of floral‐like magnetic Fe 3 O 4 @MnO 2
Author(s) -
Wang Yanying,
Zhu Xiaoyan,
Ding Fang,
Liu Yaqin,
Yang Li,
Zou Ping,
Zhao Qingbiao,
Wang Xianxiang,
Rao Hanbing
Publication year - 2019
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.3576
Subject(s) - gallic acid , absorbance , detection limit , spectroscopy , analytical chemistry (journal) , chemistry , oxidase test , materials science , nuclear chemistry , chromatography , physics , organic chemistry , enzyme , quantum mechanics , antioxidant
In this study, a colorimetric method was developed for rapid and sensitive determination of gallic acid (GA) by using floral‐like magnetic Fe 3 O 4 @MnO 2 composite material with enhanced oxidase‐like activity. Fe 3 O 4 @MnO 2 composite material is able to oxidize 3,3′,5,5′‐tetramethylbenzidine (TMB) to a blue product (oxTMB) with apparent color change and absorbance at 652 nm. GA can reduce the oxTMB yielding a fading blue color. Based on these results, a technique is proposed to detect GA quantitatively and qualitatively with UV–vis spectroscopy and bare eyes. A low detection limit of 0.105 μM and a detection range of 0.01 to 15 μM were obtained with UV–vis spectroscopy. This methodology possesses high potential for application in determination of GA.