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Dual site‐controlled two‐photon fluorescent probe for the imaging of lysosomal pH in living cells
Author(s) -
Wang Chao,
Dong Baoli,
Kong Xiuqi,
Zhang Nan,
Song Wenhui,
Lin Weiying
Publication year - 2018
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.3546
Subject(s) - lysosome , fluorescence , chemistry , fluorescence lifetime imaging microscopy , two photon excitation microscopy , biophysics , piperazine , morpholine , biochemistry , biology , medicinal chemistry , enzyme , physics , organic chemistry , quantum mechanics
Abnormal lysosomal pH is closely associated with many diseases, and real‐time monitoring of lysosomal pH is important for understanding the lysosome physiological nature. Here, we present a novel lysosome‐targeting two‐photon fluorescent probe ( MP‐lys ) for monitoring pH changes in living cells. As a dual site‐controlled probe, MP‐lys employed morpholine and piperazine groups as the lysosome‐targeting groups and pH response sites. MP‐lys showed rapid, reversible and sensitive fluorescence response to pH. MP‐lys possessed lysosome‐targeting properties, and could be used for two‐photon imaging of chloroquine‐induced pH variation in living cells.