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A sensitive resonance Rayleigh light scattering method for alpinetin using gold nanorods probes
Author(s) -
Bi Shuyun,
Sun Xiaoyue,
Wang Yu,
Wu Jun,
Zhou Huifeng
Publication year - 2018
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.3531
Subject(s) - nanorod , detection limit , rayleigh scattering , chemistry , analytical chemistry (journal) , resonance (particle physics) , materials science , chromatography , nanotechnology , optics , physics , atomic physics
A sensitive resonance Rayleigh light scattering (RLS) assay for alpinetin was developed based on alpinetin‐modified gold nanorods (AuNRs). Alpinetin could interact with AuNRs and formed a new assembly by electrostatic attraction. In pH 7.4 Tris–HCl buffer solution, the assembly of alpinetin–AuNRs showed a sensitive RLS signal. Under optimum conditions, the magnitude of enhanced RLS intensity (Δ I RLS ) was proportional to the concentration of alpinetin over the range 0.027–3.24 μg ml −1 , with a detection limit of 1.79 ng ml −1 (by 3 σ ). The developed RLS method was successfully applied to the detection of alpinetin in real or synthesized samples. Alpinetin recoveries were 90.4–108.7% with an RSD of 0.82–2.9% ( n = 5) for Alpinia katsumadai samples, and 95.1–103.7% with an RSD of 0.28–3.9% ( n = 5) for synthesized samples. The results showed that this new approach was convenient, reliable and sensitive.