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Luminescent nanoparticles for rapid monitoring of endogenous acetylcholine release in mice atria
Author(s) -
Mukhametshina Alsu,
Petrov Alexey,
Fedorenko Svetlana,
Petrov Konstantin,
Nizameev Irek,
Mustafina Asiya,
Sinyashin Oleg
Publication year - 2018
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.3450
Subject(s) - acetylcholine , acetylcholinesterase , chemistry , quenching (fluorescence) , biophysics , biochemistry , fluorescence , enzyme , pharmacology , biology , physics , quantum mechanics
The present work introduces for the first time a nanoparticulate approach for ex vivo monitoring of acetylcholinesterase‐catalyzed hydrolysis of endogenous acetylcholine released from nerve varicosities in mice atria. Amino‐modified 20‐nm size silica nanoparticles (SNs) doped by luminescent Tb(III) complexes were applied as the nanosensors. Their sensing capacity results from the decreased intensity of Tb(III)‐centred luminescence due to the quenching effect of acetic acid derived from acetylcholinesterase‐catalyzed hydrolysis of acetylcholine. Sensitivity of the SNs in monitoring acetylcholine hydrolysis was confirmed by in vitro experiments. Isolated atria were exposed to the nanosensors for 10 min to stain cell membranes. Acetylcholine hydrolysis was monitored optically in the atria samples by measuring quenching of Tb(III)‐centred luminescence by acetic acid derived from endogenous acetylcholine due to its acetylcholinesterase‐catalyzed hydrolysis. The reliability of the sensing was demonstrated by the quenching effect of exogenous acetylcholine added to the bath solution. Additionally, no luminescence quenching occurred when the atria were pre‐treated with the acetylcholinesterase inhibitor paraoxon.