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A red‐emitting indolium fluorescence probe for membranes ‐ flavonoids interactions
Author(s) -
Gao Qingyun,
Liu Han,
Ding Qiongjie,
Du Jinya,
Liu Chunlin,
Yang Wei,
Shen Ping,
Yang Changying
Publication year - 2018
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.3449
Subject(s) - liposome , chemistry , fluorescence , membrane , affinities , lipid bilayer , intramolecular force , photochemistry , stereochemistry , biochemistry , physics , quantum mechanics
The red‐emitting indolium derivative compound ( E )‐2‐(4‐(diphenylamino)styryl)‐1,3,3‐trimethyl‐3H‐indol‐1‐ium iodide (H3) was demonstrated as a sensitive membrane fluorescence probe. The probe located at the interface of liposomes when mixed showed much fluorescence enhancement by inhibiting the twisted intramolecular charge transfer state. After ultrasonic treatment, it penetrated into lipid bilayers with the emissions leveling off and a rather large encapsulation efficiency (71.4%) in liposomes. The ζ ‐potential and particle size measurement confirmed that the charged indolium group was embedded deeply into lipid bilayers. The probe was then used to monitor the affinities of antioxidant flavonoids for membranes. It was verified that quercetin easily interacted with liposomes and dissociated the probe from the internal lipid within 60 s under the condition of simply mixing. The assessment of binding affinities of six flavonoids and the coincident results with their antioxidation activities indicated that it was a promising membrane probe for the study of drug bio‐affinities.