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Quenching of graphene quantum dots fluorescence by alkaline phosphatase activity in the presence of hydroquinone diphosphate
Author(s) -
Pereira da Silva Neves Marta Maria,
GonzálezGarcía María Begoña,
PérezJunquera Alejandro,
HernándezSantos David,
FanjulBolado Pablo
Publication year - 2018
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.3445
Subject(s) - hydroquinone , fluorescence , quenching (fluorescence) , alkaline phosphatase , quantum dot , benzoquinone , graphene , chemistry , luminescence , photochemistry , enzyme , materials science , biochemistry , nanotechnology , optoelectronics , optics , physics
In this work, a turn‐off photoluminescent sensing proof‐of‐concept based on blue luminescent graphene quantum dots (GQDs) as the fluorescent probe was developed. For that purpose, GQDs optical response was related with the catalytic enzymatic activity of alkaline phosphatase (ALP), in the presence of hydroquinone diphosphate (HQDP). The hydrolysis of HQDP by ALP generated hydroquinone (HQ). The oxidation of HQ, enzymatically produced, to p ‐benzoquinone (BQ) resulted in the quenching of GQDs fluorescence (FL). Therefore, the developed luminescent sensing mechanism allowed the FL quenching with ALP activity to be related and thus quantified the concentration of ALP down to 0.5 nM of enzyme. This innovative design principle appears as a promising tool for the development of enzymatic sensors based on ALP labeling with fluorescent detection or even for direct ALP luminescent quantification in an easy, fast and sensitive manner.