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In situ biosensor for detection miRNA in living cells based on carbon nitride nanosheets with catalytic hairpin assembly amplification
Author(s) -
Liao Xianjiu,
Li Liqing,
Pan Jianbin,
Peng Tingting,
Ge Bin,
Tang Qianli
Publication year - 2018
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.3392
Subject(s) - biosensor , fluorescence , graphitic carbon nitride , nanosheet , intracellular , detection limit , nanotechnology , molecular beacon , chemistry , biophysics , dna , materials science , catalysis , biochemistry , oligonucleotide , biology , chromatography , photocatalysis , quantum mechanics , physics
In this study, an ultrasensitive fluorescence turn‐on assay for in situ sensing of intracellular microRNA (miRNA) was developed utilizing a carbon nitride nanosheet (CNNS) and a catalytic hairpin assembly (CHA). The CHA showed favourable signal amplification for low‐level biomarkers, and CNNS was an excellent candidate as a fluorescence quencher and gene vector. Moreover, the hairpin DNA of CHA could be adsorbed onto the surface of CNNS. An enzyme‐free fluorescence biosensor for ultrasensitive sensing of intracellular miRNA in cells based on CHA and CNNS was designed. When faced with target miRNA, the fluorescence was recovered due to the miRNA, which could trigger cycling of CHA circuits, leading to the production of a marked enhanced fluorescence signal. Compared with traditional methods, the proposed method is convenient, with low cytotoxicity, and high specificity and ultrasensitivity. It has promising potential for detection low‐level biomarkers.