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A sensitive surface‐enhanced Raman scattering method for chondroitin sulfate with Victoria blue 4R molecular probes in nanogold sol substrate
Author(s) -
Luo Yanghe,
Wang Xiaoliang,
Liu Qingye,
Liang Aihui,
He Xingcun,
Jiang Zhiliang
Publication year - 2018
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.3382
Subject(s) - raman scattering , chondroitin sulfate , detection limit , substrate (aquarium) , colloidal gold , chemistry , nanoparticle , raman spectroscopy , analytical chemistry (journal) , materials science , nanotechnology , chromatography , optics , biochemistry , physics , oceanography , geology , glycosaminoglycan
Using silver nanoparticles (AgNPs) as the nanocatalyst, l ‐cysteine rapidly reduced HAuCl 4 to make a stable gold nanoparticle sol (Ag/AuNP) that had a high surface‐enhanced Raman scattering (SERS) activity in the presence of Victoria blue 4R (VB4r) molecular probes. Under the selected conditions, chondroitin sulfate (Chs) reacted with the VB4r probes to form associated complexes that caused the SERS effect to decrease to 1618 cm −1 . The decreased SERS intensity was linear to the Chs concentration in the range 3.1–500 ng/ml, with a detection limit of 1.0 ng/ml Chs. Accordingly, we established a simple and sensitive SERS quantitative analysis method to determine Chs in real samples, with a relative standard deviation of 1.47–3.16% and a recovery rate of 97.6–104.2%.