Premium
Spectrofluorometric determination of clonazepam in dosage forms: Application to content uniformity testing and human plasma
Author(s) -
Belal Fathalla,
Ibrahim Fawzia,
Sheribah Zainab,
Alaa Heba
Publication year - 2017
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.3325
Subject(s) - detection limit , fluorophore , chromatography , calibration curve , fluorescence , chemistry , analytical chemistry (journal) , calibration , materials science , mathematics , optics , physics , statistics
The present paper describes a developed and validated simple, highly sensitive and cost‐effective spectrofluorometric method for determination of clonazepam (CNP). The proposed method depends on forming a highly fluorescent product through the reduction of CNP with Zn/HCl. The produced fluorophore exhibits a strong fluorescence at λ em 350 nm after excitation at λ ex 250 nm. The use of carboxymethylcellulose (CMC) greatly enhanced the fluorescence intensity of the produced fluorophore to the extent of about 100%. Calibration curve showed good linear regression ( r 2 > 0.9998) within test ranges of 20–400 ng ml −1 with a lower detection limit of 0.67 ng ml −1 and lower quantification limit of 2.22 ng ml −1 upon using CMC. The method was successfully applied to the analysis of CNP in its pharmaceutical formulations and the results were in agreement with those obtained using a reference method. Furthermore, the content uniformity testing of the tablets was also performed. The application of the proposed method was extended to determine CNP in spiked human plasma sample as a preliminary investigation and the results were satisfactory.