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A colorimetric and fluorogenic probe for bisulfite using benzopyrylium as the recognition unit
Author(s) -
Zhang Yun,
Zhang Xiangwen,
Yang XiaoFeng,
Zhang Juan
Publication year - 2017
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.3316
Subject(s) - bisulfite , fluorophore , chemistry , coumarin , detection limit , moiety , fluorescence , photochemistry , sodium bisulfite , selectivity , combinatorial chemistry , chromatography , organic chemistry , biochemistry , gene expression , physics , quantum mechanics , dna methylation , gene , catalysis
A coumarin–benzopyrylium ( CB ) platform has been developed for the colorimetric and fluorogenic detection of bisulfite. The proposed probe utilizes coumarin as the fluorophore and positively charged benzopyrylium as the reaction site. The method employs the nucleophilic addition of bisulfite to the benzopyrylium moiety of CB to inactivate the electron‐deficient oxonium ion. The driving force for photo‐induced electron transfer is considerably diminished, thereby promoting the emission intensity of the coumarin fluorophore. The fluorescence intensity at 510 nm is linear with bisulfite concentration over a range of 0.2–7.5 μM with a detection limit of 42 nM (3δ). CB shows a rapid response (within 30 s) and high selectivity and sensitivity for bisulfite. Preliminary studies show that CB has great potential for bisulfite detection in real samples and in living cells.