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Study on physisorption between G‐actin and amphiphilic polymer‐coated gold nanoparticles
Author(s) -
Du Qiqige,
Li Wanrong,
Yuan Ming,
Gong Pei,
Zhang Yi,
Zhang Feng
Publication year - 2017
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.3229
Subject(s) - physisorption , amphiphile , nanomaterials , nanoparticle , biomolecule , quenching (fluorescence) , chemistry , polymer , circular dichroism , conjugate , colloidal gold , nanotechnology , chemical engineering , biophysics , materials science , crystallography , organic chemistry , fluorescence , adsorption , copolymer , mathematical analysis , physics , mathematics , quantum mechanics , engineering , biology
Physisorptions occurs everywhere and constantly in living organisms and between nanomaterials and biomolecules. In this study, one of the most important proteins, G‐actin, was selected to investigate its bio‐nano physisorption with a model nanoparticle coated with a amphiphilic polymer. Using a photoluminescence quenching method, both the binding constant and the Hill constant were determined as 1.79 × 10 7 M −1 and 0.84, respectively. Thermodynamic calculations proved that such a physisorption was a spontaneous procedure. The physisorption‐mediated protein–nanoparticle conjugates were robust enough to resist gel electrophoresis, and protein conformation was kept intact, as revealed using circular dichroism. This conjugate might be a promising candidate for nanofabrication or could play a significant role in actin–related bioactivities.