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Spectroscopic studies of bovine serum albumin adsorbed onto magnetic–thermosensitive carbon microspheres
Author(s) -
Zhang Huan,
Chen Lin,
Li Longfei,
Yang Yongzhen,
Liu Xuguang
Publication year - 2016
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.3130
Subject(s) - bovine serum albumin , chemistry , circular dichroism , fluorescence , absorbance , quenching (fluorescence) , spectrophotometry , serum albumin , chromatography , crystallography , biochemistry , physics , quantum mechanics
Abstract To investigate the influence of magnetic–thermosensitive carbon microspheres (MTCMSs) as a targeting drug carrier on serum albumins in vitro , in this study, bovine serum albumin (BSA) was chosen as a template protein to explore the interaction between serum proteins and MTCMSs. Fluorescence spectrophotometry, ultraviolet–visible absorbance (UV–vis) spectrophotometry and circular dichroism spectrometry were used to investigate the interaction between MTCMSs and BSA. Results indicate that BSA interacts with MTCMSs and the fluorescence intensity of BSA is quenched by 50% in a static quenching at 310 K when the concentration of MTCMSs reaches 30 mg/L. Thermodynamic parameters including free energy change ( △G θ ), enthalpy change ( △H θ ) and entropy change ( △S θ ) were calculated. The results ( △G θ < 0, △H θ < 0 and △S θ > 0) suggest a spontaneous process and the formation of a hydrogen bond between MTCMSs and BSA. UV–vis measurements reveal that the micro‐environment of an amino acid residue is altered in the presence of MTCMSs. The α ‐helix content of BSA decreases by 4% and the β‐sheet content increases by 3.2% with increasing concentrations of MTCMSs to 30 mg/L, illustrating a change in the skeletal structure of BSA. These results demonstrate that MTCMSs as a targeting drug carrier impact the structure of serum albumins. This work provides not only a theoretical basis of BSA adsorption onto MTCMSs, but also an understanding of safe drug carriers in biomedicine. Copyright © 2016 John Wiley & Sons, Ltd.