Premium
A fluorescent aptasensor for sensitive analysis oxytetracycline based on silver nanoclusters
Author(s) -
Hosseini Morteza,
Mehrabi Fatemeh,
Ganjali Mohammad Reza,
Norouzi Parviz
Publication year - 2016
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.3112
Subject(s) - nanoclusters , aptamer , fluorescence , detection limit , dna , oxytetracycline , chemistry , quenching (fluorescence) , cytosine , linear range , combinatorial chemistry , nucleotide , chromatography , biophysics , biochemistry , microbiology and biotechnology , antibiotics , biology , organic chemistry , physics , quantum mechanics , gene
A fluorescent aptasensor for detection of oxytetracycline (OTC) was presented based on fluorescence quenching of DNA aptamer‐templated silver nanoclusters (AgNCs). The specific DNA scaffolds with two different nucleotides fragments were used: one was enriched with a cytosine sequence fragment (C12) that could produce DNA–AgNCs via a chemical reduction method, and another was the OTC aptamer fragment that could selectively bind to the OTC antibiotic. Thus, the as‐prepared AgNCs could exhibit quenched fluorescence after binding to the target OTC. The fluorescence ratio of the DNA–AgNCs was quenched in a linearly proportional manner to the concentration of the target in the range of 0.5 nM to 100 nM with a detection limit of 0.1 nM. This proposed nanobiosensor was demonstrated to be sensitive, selective, and simple, introducing a viable alternative for rapid determination of toxin OTC in honey and water samples. Copyright © 2016 John Wiley & Sons, Ltd.