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Biosensitive element in the form of immobilized luminescent photobacteria for detecting ecotoxicants in aqueous flow‐through systems
Author(s) -
Efremenko Ele.,
Maslova Olga V.,
Kholstov Alexander V.,
Senko Olga V.,
Ismailov Anvar D.
Publication year - 2016
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.3104
Subject(s) - chemistry , aqueous solution , photobacterium phosphoreum , luminescence , pentachlorophenol , metal ions in aqueous solution , chromatography , nuclear chemistry , metal , organic chemistry , materials science , toxicity , optoelectronics
We demonstrated the possibility of long‐term and efficient application of a biosensitive element (BE) in the form of Photobacterium phosphoreum photobacteria immobilized in poly(vinyl alcohol) (PVA) cryogel for detecting various ecotoxicants ( Zn 2 + , Cu 2 + , Hg 2 + , Pb 2 + , 2,4‐dichlorophenoxyacetic acid, 2,6‐dimethylphenol, pentachlorophenol, coumaphos, malathion, chlorpyrifos and methyl parathion) in flow‐through media. The range of detectable concentrations of ecotoxicants was determined at 1 × 10 −8 to 1 × 10 −4 M for heavy metal ions and at 1 × 10 −8 to 1 × 10 −5 M for phenol derivatives and organophosphorus pesticides. Immobilized cells of photobacteria quantitatively reacted with these ecotoxicants; cell sensitivity exhibited no flow rate dependence in the range from 45 to 180 mL/h. At a constant concentration of ecotoxicant in the flow, the bioluminescence quenching profile of immobilized cells demonstrated an integral response. The BE could remain in a flow‐through medium for at least 10 days while retaining 95% of luminescent activity in the absence of ecotoxicants. The BE tested in this work was demonstrated to have a long shelf life (> 60 weeks) at −80°C without changes in the baseline level of bioluminescence. Copyright © 2016 John Wiley & Sons, Ltd.