Premium
Studies on the antioxidant properties of some phytoestrogens
Author(s) -
Kładna Aleksandra,
Berczyński Paweł,
Kruk Irena,
Piechowska Teresa,
AboulEnein Hassan Y.
Publication year - 2016
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.3091
Subject(s) - daidzein , equol , chemistry , dpph , genistein , phytoestrogens , isoflavones , antioxidant , biochanin a , radical , hydroxyl radical , chemiluminescence , nuclear chemistry , biochemistry , organic chemistry , medicine , estrogen
Isoflavones genistein and daidzein are nonsteroidal phytoestrogens occurring mainly in soybean foods. These phytoestrogens possess estrogenic properties and show a variety of health benefits as anti‐inflammatory agents. However, the mechanism of their action has not been identified in detail. The aim of this study is to characterize the antioxidant powers of genistein, daidzein and daidzein metabolite–equol through their activities to scavenge superoxide anion radical (O • 2 − ), hydroxyl radical (HO • ), 2,2–diphenyl–1‐picrylhydrazyl radical (DPPH • ) and hydrogen peroxide (H 2 O 2 ) using chemiluminescence and spectrophotometry techniques. Potassium superoxide in dimethyl sulphoxide (DMSO) and 18‐crown‐6 ether were used as a source of O • 2 − . Hydroxyl radicals were produced using the Fenton reaction. In free radical assays, genistein had the IC 50 values (an amount of antioxidant concentration required to decrease the initial radical concentration by 50%) 0.391 ± 0.012 mM for O • 2 − , 0.621 ± 0.028 mM for HO • and 1.89 ± 0.16 mM for DPPH • . The IC 50 values for daidzein for these free radicals were 1.924 ± 0.011 mM, 0.702 ± 0.012 mM and 2.81 ± 0.03 mM, respectively. Equol was the most active the free radical scavenger with IC 50 = 0.451 ± 0.018 mM for HO • and IC 50 = 1.36 ± 0.11 mM for DPPH • . All tested compounds exerted a significant effect on the H 2 O 2 : IC 50 = 18.1 ± 1.1 μM for genistein, IC 50 = 2.1 ± 0.5 μM for daidzein, and IC 50 = 1.06 ± 0.2 μM for equol. These findings show that genistein, daidzein and equol are effective free radical scavengers and possess high antioxidant power in vitro . Copyright © 2016 John Wiley & Sons, Ltd.