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A sensitive fluorescence method for detection of E. Coli using rhodamine 6G dyeing
Author(s) -
Wang Yaohui,
Jiang Caina,
Wen Guiqing,
Zhang Xinghui,
Luo Yanghe,
Qin Aimiao,
Liang Aihui,
Jiang Zhiliang
Publication year - 2016
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.3060
Subject(s) - bacillus subtilis , rhodamine 6g , fluorescence , chemistry , staphylococcus aureus , escherichia coli , detection limit , rhodamine b , rhodamine , chromatography , bacteria , nuclear chemistry , biology , biochemistry , organic chemistry , molecule , genetics , physics , quantum mechanics , photocatalysis , gene , catalysis
Negatively charged bacteria combined with positively charged alkaline dye rhodamine 6G (Rh6G) in NaH 2 PO 4 –Na 2 HPO 4 buffer solution pH 7.4, by electrostatic interaction. The dyed bacteria exhibited a strong fluorescence peak at 552 nm and fluorescence intensity was directly linear to Escherichia coli ( E. coli ), Bacillus subtilis ( B. subtilis ) and Staphylococcus aureus ( S. aureus ) concentrations in the range of 7.06 × 10 4 to 3.53 × 10 7 , 4.95 × 10 5 to 2.475 × 10 8 and 32.5 to 16250 colony forming unit/mL (cfu/mL) respectively, with detection limits of 3.2 × 10 4 cfu/mL E. coli , 2.3 × 10 5 cfu/mL B. subtilis and 16 cfu/mL S. aureus , respectively. Samples were cultured for 12 h, after which the linear detection range for E. coli was 2 to 88 cfu/mL. This simple, rapid and sensitive method was used for the analysis of water and drinking samples. Copyright © 2015 John Wiley & Sons, Ltd.
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