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BSA‐conjugated zinc oxide nanoparticles as luminescent probes for the determination of histidine
Author(s) -
Wu Dudu,
Liang Qiaowen,
Chen Zhi
Publication year - 2016
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.3059
Subject(s) - histidine , bovine serum albumin , chemistry , zinc , quenching (fluorescence) , detection limit , nanoparticle , fluorescence , aqueous solution , conjugated system , luminescence , nuclear chemistry , photochemistry , amino acid , inorganic chemistry , chromatography , materials science , nanotechnology , organic chemistry , biochemistry , polymer , physics , optoelectronics , quantum mechanics
Zinc oxide nanoparticles doped with bovine serum albumin were used to determine histidine in aqueous solutions using a fluorescence spectroscopic technique. The results showed that histidine effectively quenched the fluorescence of the modified ZnO nanoparticles, whereas other amino acids did not significantly affect the light emission, thereby allowing selective and sensitive histidine detection in amino acid mixtures. Under optimal conditions (pH 7.0, 25 °C, 10 min preincubation), the detection limit for histidine was ~ 9.87 × 10 –7 mol/L. The high value of the determined quenching rate constant K q (3.30 × 10 13 L/mol/s) was consistent with a static quenching mechanism. Copyright © 2015 John Wiley & Sons, Ltd.