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A water‐soluble fluorescent pH probe based on perylene dyes and its application to cell imaging
Author(s) -
Ma Yongshan,
Zhang Fengxia,
Zhang Jinfeng,
Jiang Tianyi,
Li Xuemei,
Wu Junsen,
Ren Huixue
Publication year - 2016
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.2930
Subject(s) - perylene , fluorescence , quantum yield , chemistry , hela , amine gas treating , solubility , photochemistry , yield (engineering) , aqueous solution , organic chemistry , materials science , molecule , cell , optics , biochemistry , physics , metallurgy
Abstract A fluorescent pH probe, N , N ′‐bi( l ‐phenylalanine amine)‐perylene‐3,4;9,10‐dicarboxylic diimide (PDCDA) was synthesized and used for pH sensing in living cells. A significant fluorescence intensity change was observed over a pH range from 7.0 to 4.0. Electrostatic potential maps (MEP) suggested that the electronic repulsion between PDCDAs was increased by the high negative electrostatic potential which resulted in a high water solubility of PDCDA. PDCDA was successfully applied as a high‐performance fluorochrome for living HeLa cell imaging. The results demonstrate that the probe PDCDA is a good candidate for monitoring pH fluctuations in living cells with good water solubility, low cytotoxicity, high fluorescence quantum yield and photostability. Copyright © 2015 John Wiley & Sons, Ltd.