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Sensitive fluorimetric assays for α‐glucosidase activity and inhibitor screening based on β‐cyclodextrin‐coated quantum dots
Author(s) -
Liu SiYao,
Wang Huan,
He Tian,
Qi Liang,
Zhang ZhiQi
Publication year - 2016
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.2929
Subject(s) - cyclodextrin , acarbose , quantum dot , fluorescence , chemistry , hydrolysis , combinatorial chemistry , chromatography , enzyme , nanotechnology , biochemistry , materials science , physics , quantum mechanics
A fluorescence method was established for a α‐glucosidase activity assay and inhibitor screening based on β‐cyclodextrin‐coated quantum dots. p ‐Nitrophenol, the hydrolysis product of the α‐glucosidase reaction, could quench the fluorescence of β‐cyclodextrin‐coated quantum dots via an electron transfer process, leading to fluorescence turn‐off, whereas the fluorescence of the system turned on in the presence of α‐glucosidase inhibitors. Taking advantage of the excellent properties of quantum dots, this method provided a very simple, rapid and sensitive screening method for α‐glucosidase inhibitors. Two α‐glucosidase inhibitors, 2,4,6‐tribromophenol and acarbose, were used to evaluate the feasibility of this screening model, and IC 50 values of 24 μM and 0.55 mM were obtained respectively, which were lower than those previously reported. The method may have potential application in screening α‐glucosidase inhibitors. Copyright © 2015 John Wiley & Sons, Ltd.