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Utility of gold nanoparticles in luminescence determination of trovafloxacin: comparison of chemiluminescence and fluorescence detection
Author(s) -
Alarfaj Nawal A.,
ElTohamy Maha F.
Publication year - 2015
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.2914
Subject(s) - trovafloxacin , detection limit , chemiluminescence , chemistry , luminol , linear range , fluorescence , nuclear chemistry , analytical chemistry (journal) , chromatography , nanoparticle , luminescence , antibacterial agent , materials science , nanotechnology , biochemistry , physics , quantum mechanics , antibiotics , optoelectronics
Two novel sensitive sequential injection chemiluminescence analysis and fluorescence methods for trovafloxacin mesylate detection have been developed. The methods were based on the enhancement effect of gold nanoparticles on luminol–ferricyanide–trovafloxacin and europium(III)–trovafloxacin complex systems. The optimum conditions for both detection methods were investigated. The chemiluminescence signal was emitted due to the enhanced effect of gold nanoparticles on the reaction of luminol–ferricyanide–trovafloxacin in an alkaline medium. The response was linear over a concentration range of 1.0 × 10 –9 to 1.0 × 10 –2 mol/L (%RSD = 1.3), ( n = 9, r = 0.9991) with a detection limit of 1.7 × 10 –10 mol/L (S/N = 3). The weak fluorescence intensity signal of the oxidation complex of europium(III)–trovafloxacin was strongly enhanced by gold nanoparticles and detected at λ ex = 330 and λ em = 540 nm. Fluorescence detection enabled the determination of trovafloxacin mesylate over a linear range of 1.0 × 10 –8 to 1.0 × 10 –3 mol/L (%RSD = 1.2), ( n = 6, r = 0.9993) with a detection limit of 3.3 × 10 –9 mol/L. The proposed methods were successfully applied to the determination of the studied drug in its bulk form and in pharmaceutical preparations. The results were treated statistically and compared with those obtained from other reported methods. Copyright © 2015 John Wiley & Sons, Ltd.