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Highly sensitive fluorescence detection of glycoprotein based on energy transfer between CuInS 2 QDs and rhodamine B
Author(s) -
Gao Xue,
Li Dan,
Tong Ying,
Ge Dan,
Tang Yiwei,
Zhang Defu,
Li Jianrong
Publication year - 2015
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.2911
Subject(s) - förster resonance energy transfer , detection limit , fluorescence , chemistry , acceptor , quantum dot , linear range , lectin , rhodamine , analytical chemistry (journal) , photochemistry , biophysics , chromatography , biochemistry , materials science , nanotechnology , physics , quantum mechanics , biology , condensed matter physics
A highly sensitive fluorescence method for glycoprotein detection has been established based on fluorescence resonance energy transfer (FRET) between CuInS 2 quantum dots (QDs) and rhodamine B (RB). Lectins comprise a group of proteins with unique affinities toward carbohydrate structures, so the process of FRET can occur between lectin‐coated QDs (CuInS 2 QDs–Con A conjugates, acceptors) and carbohydrate‐coated RB (RB–NH 2 ‐glu conjugates, donors). The fluorescence of lectin‐coated QDs was recovered in the presence of a glycoprotein such as glucose oxidase (GOx) and transferrin (TRF), which significantly reduced the FRET efficiency between the donor and the acceptor. Under optimal conditions, a linear correlation was established between the fluorescence intensity ratio I 654 / I 577 and the TRF concentration over the range of 6.90 × 10 ‐10 to 3.45 × 10 ‐8 mol/L, with a detection limit of 2.5 × 10 ‐10 mol/L. The linear range for GOx is 3.35 × 10 ‐10 to 6.70 × 10 ‐8 mol/L, with a detection limit of 1.5 × 10 ‐10 mol/L. The proposed method was applied to the determination of glycoprotein in human serum and cell‐extract samples with satisfactory results. Furthermore, CuInS 2 QDs–Con A conjugates are used as safe and efficient optical nanoprobes in HepG2 cell imaging. Copyright © 2015 John Wiley & Sons, Ltd.