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Utility of eosin Y as a complexing reagent for the determination of citalopram hydrobromide in commercial dosage forms by fluorescence spectrophotometry
Author(s) -
Azmi Syed Najmul Hejaz,
AlFazari Ahlam,
AlBadaei Munira,
AlMahrazi Ruqiya
Publication year - 2015
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.2905
Subject(s) - chemistry , detection limit , chromatography , reagent , calibration curve , dosage form , fluorescence , citric acid , spectrophotometry , eosin , staining , biochemistry , medicine , physics , quantum mechanics , pathology
An accurate, selective and sensitive spectrofluorimetric method was developed for the determination of citalopram hydrobromide in commercial dosage forms. The method was based on the formation of a fluorescent ion‐pair complex between citalopram hydrobromide and eosin Y in the presence of a disodium hydrogen phosphate/citric acid buffer solution of pH 3.4 that was extractable in dichloromethane. The extracted complex showed fluorescence intensity at λ em = 554 nm after excitation at 259 nm. The calibration curve was linear over at concentrations of 2.0–26.0 µg/mL. Under optimized experimental conditions, the proposed method was validated as per ICH guidelines. The effect of common excipients used as additives was tested and the tolerance limit calculated. The limit of detection for the proposed method was 0.121 μg/mL. The proposed method was successfully applied to the determination of citalopram hydrobromide in commercial dosage forms. The results were compared with the reference RP‐HPLC method. Copyright © 2015 John Wiley & Sons, Ltd.