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Multi‐spectroscopic methods investigation on the interaction of tenoxicam with DNA
Author(s) -
Liu BingMi,
Zhang Jun,
Liu Yang,
Zhang LiPing,
Ma Ping,
Wang Xin,
Liu Bin
Publication year - 2015
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.2899
Subject(s) - tenoxicam , dna , van der waals force , chemistry , hydrogen bond , binding constant , biophysics , binding site , crystallography , molecule , biochemistry , biology , medicine , organic chemistry , pathology , alternative medicine , piroxicam
Abstract Non‐steroidal anti‐inflammatory drugs (NSAIDs) show chemopreventive and chemosuppressive effects on various cancer cell lines. They exert anticancer activities by inhibiting both at the protein level and/or at the transcription level. Thus, in this paper, the interaction between tenoxicam (TXM) and calf thymus DNA (ct‐DNA) was investigated by UV–visible light, fluorescence, viscosity experiments and DNA melting studies. The results showed that TXM could bind to ct‐DNA in the groove binding mode. The binding constants were 7.67 × 10 3 and 5.48 × 10 3 M −1 at 293 and 300 K, respectively. Furthermore, the calculated thermodynamic parameters suggested that hydrogen bonds or van der Waals force might play an important role in the binding of TXM to ct‐DNA. The obtained results should give new insight into the pharmacological activity of TXM. Copyright © 2015 John Wiley & Sons, Ltd.