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Multiplexed DNA detection using a gold nanorod‐based fluorescence resonance energy transfer technique
Author(s) -
Wu Qiang,
He Yanlong,
Tian Jianniao,
Zhang Juanni,
Hu Kun,
Zhao Yanchun,
Zhao Shulin
Publication year - 2015
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.2885
Subject(s) - multiplex , detection limit , förster resonance energy transfer , analyte , nanorod , multiplexing , fluorescence , energy transfer , materials science , analytical chemistry (journal) , chemistry , nanotechnology , computer science , chromatography , physics , optics , bioinformatics , telecommunications , biology , molecular physics
Abstract A fluorescence resonance energy transfer method for multiplex detection DNA based on gold nanorods had been successfully constructed. This method is simple, easy to operate, good selectivity, no requirement to label the probe molecule and can analyze simultaneously multiple targets of DNA in one sample. The limit of detection for the 18‐mer, 27‐mer and 30‐mer targets is 0.72, 1.0 and 0.43 nM at a signal‐to‐noise ratio of 3. The recoveries of three targets were 96.57–98.07%, 99.12–100.04% and 97.29–99.93%, respectively. The results show that the method can be used to analyze a clinical sample or a biological sample; it also can be used to develop new probes for rapid, sensitive and highly selective multiplex detection of analytes in real samples. Copyright © 2015 John Wiley & Sons, Ltd.