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Study on interactions of aminoglycoside antibiotics with calf thymus DNA and determination of calf thymus DNA via the resonance Rayleigh scattering technique
Author(s) -
Qiao Man,
Li Chunyan,
Shi Ying,
Liu Shaopu,
Liu Zhongfang,
Hu Xiaoli
Publication year - 2015
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.2876
Subject(s) - kanamycin , chemistry , aminoglycoside , resonance (particle physics) , rayleigh scattering , dna , circular dichroism , spectral line , neomycin , tobramycin , spectroscopy , detection limit , analytical chemistry (journal) , nuclear magnetic resonance , gentamicin , antibiotics , chromatography , crystallography , biochemistry , optics , physics , atomic physics , quantum mechanics , astronomy
A simple and sensitive resonance Rayleigh scattering (RRS) spectra method was developed for the determination of calf thymus DNA (ctDNA). The enhanced RRS signals were based on the interactions between ctDNA and aminoglycoside antibiotics (AGs) including kanamycin (KANA), tobramycin (TOB), gentamicin (GEN) and neomycin (NEO) in a weakly acidic medium (pH 3.3–5.7). Parameters influencing the method were investigated. Under optimum conditions, increments in the scattering intensity (∆ I ) were directly proportional to the concentration of ctDNA over certain ranges. The detection limit ranged from 12.2 to 16.9 ng/mL. Spectroscopic methods, including RRS spectra, absorption spectra and circular dichroism (CD) spectroscopy, coupled with thermo‐denaturation experiments were used to study the interactions, indicating that the interaction between AGs with ctDNA was electrostatic binding mode. Copyright © 2015 John Wiley & Sons, Ltd.