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Assay of ceftazidime and cefepime based on fluorescence quenching of carbon quantum dots
Author(s) -
Huang Yu,
Zhang Ying,
Yan Zhengyu,
Liao Shenghua
Publication year - 2015
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.2871
Subject(s) - cefepime , quenching (fluorescence) , ceftazidime , fourier transform infrared spectroscopy , fluorescence , chemistry , ethylene glycol , analytical chemistry (journal) , aqueous solution , nuclear chemistry , photochemistry , chromatography , organic chemistry , chemical engineering , genetics , physics , quantum mechanics , bacteria , engineering , pseudomonas aeruginosa , biology
A novel and sensitive method for the determination of ceftazidime and cefepime in an active pharmaceutical ingredient (API) has been developed based on the fluorescence quenching of poly(ethylene glycol) (PEG)2000‐capped carbon quantum dots (CQDs) prepared using a chemical oxidation method. The quenching of fluorescence intensity is proportional to the concentration of ceftazidime and cefepime over the range of 0.33–3.30 and 0.24–2.40 µg/mL, respectively. The mode of interaction between PEG2000‐capped CQDs and ceftazidime/cefepime in aqueous solutions was investigated using a fluorescence, UV/Vis and Fourier transform infrared spectrometry (FTIR) at physiological pH. UV/Vis and FTIR spectra demonstrated that ground state compounds were formed through hydrophobic interaction the fluorescence quenching of CQDs caused by ceftazidime and cefepime. The quenching constants decreased with increases in temperature, which was consistent with static quenching. Copyright © 2015 John Wiley & Sons, Ltd.