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The preparation of ethylenediamine‐modified fluorescent carbon dots and their use in imaging of cells
Author(s) -
Dong Wei,
Zhou Siqi,
Dong Yan,
Wang Jingwen,
Ge Xin,
Sui Lili
Publication year - 2015
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.2834
Subject(s) - ethylenediamine , fluorescence , carcinoembryonic antigen , cytotoxicity , fourier transform infrared spectroscopy , chemistry , nuclear chemistry , photoluminescence , materials science , inorganic chemistry , chemical engineering , biochemistry , optoelectronics , cancer , medicine , optics , in vitro , physics , engineering
In this work, fluorescent carbon dots (CDs) were synthesized using a hydrothermal method with glucose as the carbon source and were surface‐modified with ethylenediamine. The properties of as‐prepared CDs were analyzed by transmission electron microscopy (TEM), Fourier transform infrared (FTIR), ultraviolet–visible light (UV/vis) absorption and fluorescent spectra. Furthermore, CDs conjugated with mouse anti‐(human carcinoembryonic antigen) (CEA) monoclonal antibody were successful employed in the biolabeling and fluorescent imaging of human gastric carcinoma cells. In addition, the cytotoxicity of CDs was also tested using human gastric carcinoma cells. There was no apparent cytotoxicity on human gastric carcinoma cells. These results suggest the potential application of the as‐prepared CDs in bioimaging and related fields. Copyright © 2015 John Wiley & Sons, Ltd.