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A novel system of galangin–potassium permanganate–polyphosphoric acid for the determination of tryptophan and its chemiluminescence mechanism
Author(s) -
Li Li,
Guo Ruibin,
Zhang Dongxia,
Du Xinzhen
Publication year - 2015
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.2769
Subject(s) - chemiluminescence , chemistry , galangin , tryptophan , potassium permanganate , detection limit , luminescence , chromatography , amino acid , inorganic chemistry , flavonoid , organic chemistry , biochemistry , kaempferol , physics , optoelectronics , antioxidant
A novel galangin–potassium permanganate (KMnO 4 )–polyphosphoric acid (PPA) system was found to have an outstanding response to tryptophan (Trp). Trp determination using this KMnO 4 –PPA system was enhanced significantly in the presence of galangin. A highly sensitive flow‐injection chemiluminescence (CL) method to determine Trp was developed based on the CL reaction of galangin–KMnO 4 –Trp in PPA media. The presence of galangin, a member of the flavonol class of flavonoid complexes, greatly increased the luminous intensity of Trp in KMnO 4 –PPA systems. Under optimized conditions, Trp was determined in the 0.05–10 µg/mL range, with a detection limit (3σ) of 5.0 × 10 −3 µg/mL. The relative standard deviation (RSD) was 1.0% for 11 replicate determinations of 1.0 µg/mL Trp. Two synthetic samples were determined selectively with recoveries of 98.4–100.1% in the presence of other amino acids. The possible mechanism is summarized as follows: excited states of Mn(II) * and Mn(III * types are the main means of generating chemical luminescent species, and Trp concentration and luminescence intensity have a linear relationship, which enables quantitative analysis. Copyright © 2014 John Wiley & Sons, Ltd.