Premium
Host–guest inclusion complex of mesalazine and β‐cyclodextrin and spectrofluorometric determination of mesalazine
Author(s) -
Elbashir Abdalla A.,
Altayib Alasha Abdalla Fatima,
AboulEnein Hassan Y.
Publication year - 2015
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.2758
Subject(s) - mesalazine , molar absorptivity , absorbance , chemistry , aqueous solution , cyclodextrin , analytical chemistry (journal) , fluorescence , stability constants of complexes , rhodamine b , nuclear chemistry , chromatography , organic chemistry , optics , medicine , physics , disease , pathology , quantum mechanics , ulcerative colitis , photocatalysis , catalysis
The supramolecular interaction of mesalazine (MSZ) and β‐cyclodextrin (β‐CD) has been examined by ultraviolet–visible (UV–vis) light, infra‐red (IR) light and fluorescence spectroscopy. The formation of an inclusion complex has been confirmed based on the changes of the spectral properties. MSZ–β‐CD host–guest complex was formed in (1:1) stoichiometry and the inclusion constant (K = 1.359 × 10 2 L mol –1 ) was ascertained by typical double reciprocal plots. Furthermore, the thermodynamic parameters (ΔG°, ΔH° and ΔS°) of (MSZ–β‐CD) were obtained. Based on the remarkable enhancement of the fluorescence intensity of MSZ produced through complex formation, a simple, accurate, rapid and highly sensitive spectrofluorometric method for the determination of MSZ in aqueous solution in the presence of β‐CD was developed. The measurement of relative fluorescence intensity was carried with excitation at 330 nm and emission 493 nm. All variables affecting the reactions were studied and optimized. Beer's law was obeyed in the concentration range 0.1–0.45 µg/mL. Absorbance was found to increase linearly with increasing concentration of MSZ, which is corroborated by the calculated correlation coefficient values of 0.99989. The molar absorptivity, Sandell's sensitivity, detection and quantification limits were calculated. The validity of the described methods was assessed, and the method was successfully applied to the determination of MSZ in its pharmaceutical formulation. In addition, a solid inclusion complex was synthesized by co‐precipitation method. Copyright © 2014 John Wiley & Sons, Ltd.