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Comparison of interactions between human serum albumin and silver nanoparticles of different sizes using spectroscopic methods
Author(s) -
Zhang Wanju,
Zhang Qingbo,
Wang Fang,
Yuan Lian,
Xu Ziqiang,
Jiang Fenglei,
Liu Yi
Publication year - 2015
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.2748
Subject(s) - circular dichroism , human serum albumin , fluorescence spectroscopy , fluorescence , chemistry , spectroscopy , quenching (fluorescence) , binding constant , absorption spectroscopy , analytical chemistry (journal) , absorption (acoustics) , fluorescence correlation spectroscopy , ultraviolet visible spectroscopy , crystallography , chromatography , binding site , materials science , molecule , biochemistry , organic chemistry , physics , quantum mechanics , composite material
Abstract Three different sizes (15.9 ± 2.1 nm, 26.4 ± 3.2 nm and 39.8 ± 4.0 nm, respectively) of citrate‐coated silver nanoparticles (SNPs) have been synthesized and characterized. The interactions of the synthesized SNPs with human serum albumin (HSA) at physiological pH have been systematically studied by UV‐vis absorption spectroscopy, fluorescence spectroscopy, synchronous fluorescence spectroscopy, three‐dimensional fluorescence spectroscopy and circular dichroism (CD) spectroscopy. The results indicate that the SNPs can bind to HSA with high affinity and quench the intrinsic fluorescence of HSA. The binding constants and quenching rate constants were calculated. The apparent association constants ( K app ) values are 2.14 × 10 4 M –1 for 15.9 nm SNP, 1.65 × 10 4 M –1 for 26.4 nm SNP and 1.37 × 10 4 M –1 for 39.8 nm SNP, respectively. The values of binding constant obtained from the fluorescence quenching data match well with that determined from the absorption spectral changes. These results suggest that the smaller SNPs have stronger interactions to HSA than the larger ones at the same concentrations. Synchronous fluorescence, three‐dimensional fluorescence and CD spectroscopy studies show that the synthesized SNPs can induce slight conformational changes in HSA. Copyright © 2014 John Wiley & Sons, Ltd.