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Design of highly sensitive phosphorescence sensor for determination of procaterol hydrochloride based on inhibition of KClO 3 oxidation fluorescein isothiocyanate
Author(s) -
Liu JiaMing,
Huang Qitong,
Liu Zhenbo,
Zhang LiHong,
Zheng ZhiYong,
Lin ChangQing
Publication year - 2015
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.2743
Subject(s) - phosphorescence , detection limit , chemistry , linear range , fluorescein isothiocyanate , hydrochloride , chromatography , analytical chemistry (journal) , fluorescence , organic chemistry , physics , quantum mechanics
Procaterol hydrochloride (Prh) can inhibit KClO 3 oxidation of fluorescein isothiocyanate (FITC) to form a non‐phosphorescent compound, which causes room temperature phosphorescence (RTP) of FITC in the system to enhance sharply the linear relationship between ∆ I p and the Prh content. Thus, a rapid response and highly sensitive phosphorescence sensor for the determination of Prh has been developed based on the inhibiting effect of Prh on KClO 3 oxidation of FITC. This simple, high sensitivity (detection limit (LD) calculated by 3S b /k was 0.019 fg/spot, sample volume 0.40 µl, corresponding concentration 4.8 × 10 –14 g ml –1 ) and selective sensor with a wide linear range (0.080–11.20 g/spot) has been applied to detect Prh in blood samples, and the results were consistent with those obtained by high‐performance liquid chromatography (HPLC). Simultaneously, the mechanism of the phosphorescence sensor for the detection of Prh was also investigated using infrared spectroscopy. Copyright © 2014 John Wiley & Sons, Ltd.