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Determination of selenium via the fluorescence quenching effect of selenium on hemoglobin‐catalyzed peroxidative reaction
Author(s) -
Chen YaHong,
Zhang YaNan,
Tian FengShou
Publication year - 2015
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.2723
Subject(s) - selenium , calibration curve , chemistry , detection limit , quenching (fluorescence) , hemoglobin , fluorescence , catalysis , relative standard deviation , linear range , analytical chemistry (journal) , chromatography , biochemistry , organic chemistry , physics , quantum mechanics
A new method for the determination of selenium based on its fluorescence quenching on the hemoglobin‐catalyzed reaction of H 2 O 2 and l ‐tyrosine has been established. The effect of pH, foreign ions and the optimization of variables on the determination of selenium was examined. The calibration curve was found to be linear between the fluorescence quenching ( F 0 /F ) and the concentration of selenium within the range of 0.16‐4.00 µg/mL. The detection limit was 1.96 ng/mL and the relative standard deviation was 3.14%. This method can be used for the determination of selenium in Se‐enriched garlic bulbs with satisfactory results. Copyright © 2014 John Wiley & Sons, Ltd.