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Response surface optimized peroxyoxalate chemiluminescence of octahydro‐Schiff base derivative as new luminophor and study of the quenching effect of some cations, amino acids and cholesterol
Author(s) -
Yeganeh Faal Ali,
Jamalyan Bahare,
Bordbar Maryam,
Shayeste Tavakol Heidary,
SalavatiNiasari Masoud
Publication year - 2014
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.2662
Subject(s) - peroxyoxalate , chemiluminescence , quenching (fluorescence) , derivative (finance) , reagent , imidazole , histidine , schiff base , chemistry , nuclear chemistry , polymer chemistry , organic chemistry , fluorescence , physics , quantum mechanics , financial economics , economics , enzyme
We report the first detailed study of the characteristics of an octahydro‐Schiff base derivative as a new luminophor in the peroxyoxalate chemiluminescence (POCL) system. The effect of reagents on this new POCL system was investigated. In addition, the response surface methodology was used for evaluating the relative significance of variables in this POCL system, establishing models and determining optimal conditions. The quenching effect of some cations and compounds such as Cu 2+ , Fe 3+ , Hg 2+ , imidazole, histidine and cholesterol on an optimized POCL reaction were studied. The dynamic ranges were up to approximaterly 100 and 175 × 10 −6 M for Cu 2+ and cholesterol respectively. The detection limits were 3.3 × 10 −6 m and 2.58 × 10 −6 m for Cu 2+ and histidine, respectively. In all cases the relative standard deviations were 4–5% ( n = 4). Copyright © 2014 John Wiley & Sons, Ltd.