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Flow injection chemiluminescence determination of lercanidipine based on N ‐chlorosuccinimide–eosin Y post‐chemiluminescence reaction
Author(s) -
Wang Guowei,
Zhao Fang,
Gao Ying
Publication year - 2014
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.2651
Subject(s) - chemiluminescence , chemistry , chromatography , eosin y , eosin , flow injection analysis , detection limit , organic chemistry , medicine , staining , catalysis , photocatalysis , pathology
A novel post‐chemiluminescence (PCL) reaction was discovered when lercanidipine was injected into the CL reaction mixture of N ‐chlorosuccinimide with alkaline eosin Y in the presence of cetyltrimethylammonium bromide (CTAB), where eosin Y was used as the CL reagent and CTAB as the surfactant. Based on this observation, a simple and highly sensitive PCL method combined with a flow injection (FI) technique was developed for the assay of lercanidipine. Under optimum conditions, the CL signal was linearly related to the concentration of lercanidipine in the range 7.0 × 10 ‐10 to 3.0 × 10 ‐6 g/mL with a detection limit of 2.3 × 10 ‐10 g/mL (3σ). The relative standard deviation (RSD) was 2.1% for 1.0 × 10 ‐8 g/mL lercanidipine ( n = 13). The proposed method had been applied to the estimation of lercanidipine in tablets and human serum samples with satisfactory results. The possible CL mechanism is also discussed briefly. Copyright © 2014 John Wiley & Sons, Ltd.