Flow injection chemiluminescence determination of lercanidipine based on N ‐chlorosuccinimide–eosin Y post‐chemiluminescence reaction

A novel post‐chemiluminescence (PCL) reaction was discovered when lercanidipine was injected into the CL reaction mixture of N ‐chlorosuccinimide with alkaline eosin Y in the presence of cetyltrimethylammonium bromide (CTAB), where eosin Y was used as the CL reagent and CTAB as the surfactant. Based on this observation, a simple and highly sensitive PCL method combined with a flow injection (FI) technique was developed for the assay of lercanidipine. Under optimum conditions, the CL signal was linearly related to the concentration of lercanidipine in the range 7.0 × 10 ‐10 to 3.0 × 10 ‐6  g/mL with a detection limit of 2.3 × 10 ‐10  g/mL (3σ). The relative standard deviation (RSD) was 2.1% for 1.0 × 10 ‐8  g/mL lercanidipine ( n  = 13). The proposed method had been applied to the estimation of lercanidipine in tablets and human serum samples with satisfactory results. The possible CL mechanism is also discussed briefly. Copyright © 2014 John Wiley & Sons, Ltd.