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Selective turn‐on fluorescence assay of 6–thioguanine by using harmine‐modified silver nanoparticles
Author(s) -
Amjadi Mohammad,
Farzampour Leila
Publication year - 2014
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.2612
Subject(s) - harmine , förster resonance energy transfer , chemistry , fluorescence , silver nanoparticle , detection limit , linear range , turn (biochemistry) , nuclear chemistry , nanoparticle , analytical chemistry (journal) , photochemistry , chromatography , nanotechnology , materials science , biochemistry , physics , quantum mechanics , neuroscience , biology
ABSTRACT This article reports on a novel fluorescence resonance energy transfer (FRET) system between harmine and silver nanoparticles (AgNPs), in which harmine acts as the donor and AgNPs act as the acceptor. As a result of FRET, harmine fluorescence is quenched efficiently with a corresponding Stern–Volmer constant of 3.61 × 10 11 L/mol. It was found that upon addition of the anticancer drug, 6–thioguanine (6–TG), the fluorescence was recovered due to the competitive adsorption of this compound onto AgNPs. Based on this effect, a selective turn‐on fluorescence sensor was developed for the determination of 6–TG. Under optimum conditions, the enhanced fluorescence intensity displays a linear relationship with the concentration of 6–TG in the range 1.5 × 10 ‐8 –7.5 × 10 ‐7 M with a detection limit of 9.7 nM. The developed method was applied to the determination of this drug in a pharmaceutical preparation and human plasma samples. Copyright © 2013 John Wiley & Sons, Ltd.